南京师范大学的多位学者在美国权威杂志Analytical Chemistry 上联合发表了题为《Simultaneous Detection of Ochratoxin A and Fumonisin B1 in Cereal Samples Using an Aptamer−Photonic Crystal Encoded Suspension Array》的文章,实验所用OTA标准物质,FB1 FB2,OTA ELISA试剂盒购自华安麦科。

 

ABSTRACT:A simple, new aptamerphotonic crystal encoded suspension array was designed to simultaneously quantify and qualify ochratoxin A(OTA) and fumonisin B1(FB1) in cereal samples. The aptamers of OTA and FB1 were immobilized on the surfaces of photonic crystals by chemical bonding. When the target mycotoxins appear in a sample, the fluorescence-labeled complementary DNA of the aptamer dissociates from their double DNA hybrid and results in an obvious decrease in fluorescence intensity of the microsphere. The difference value of fluorescent intensities for each kind of silica photonic crystal microsphere (SPCM) quantitatively conveys the concentration of mycotoxin, and the structure colors or reflectance peak positions of the SPCMs confirm the kind of mycotoxin detected. The reaction conditions including the immobilization method for aptamers, hybridization, and incubation conditions have been optimized. This developed method displayed a wide linear detection range (0.011 ng/mL for OTA and 0.0011 ng/mL for FB1) and a low limit of detection (0.25 pg/mL for OTA and 0.16 pg/mL for FB1). The recovery rates in the spiked cereal samples ranged from 81.80% to 116.38% for OTA and 76.58%114.79% for FB1. The positive detection results in the naturally contaminated cereal samples were in agreement with those of classic enzyme-linked immunosorbent assay (ELISA). This simple suspension array scheme displays a great application potential for the high throughput screen assay of mycotoxins

 

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脱氧雪腐镰刀菌烯醇胶体金检测试纸条的研制及应用——《粮食与食品工业》
第四届全国粮食行业职业技能竞赛国赛用DON ELISA试剂盒操作视图

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2015-01-19

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